Intravital Imaging to Monitor Therapeutic Response in Moving Hypoxic Regions Resistant to PI3K Pathway Targeting in Pancreatic Cancer.
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ABSTRACT:
SUBMITTER: Conway JRW
PROVIDER: S-EPMC6019737 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
Intravital Imaging to Monitor Therapeutic Response in Moving Hypoxic Regions Resistant to PI3K Pathway Targeting in Pancreatic Cancer.
Conway James R W JRW Warren Sean C SC Herrmann David D Murphy Kendelle J KJ Cazet Aurélie S AS Vennin Claire C Shearer Robert F RF Killen Monica J MJ Magenau Astrid A Mélénec Pauline P Pinese Mark M Nobis Max M Zaratzian Anaiis A Boulghourjian Alice A Da Silva Andrew M AM Del Monte-Nieto Gonzalo G Adam Arne S A ASA Harvey Richard P RP Haigh Jody J JJ Wang Yingxiao Y Croucher David R DR Sansom Owen J OJ Pajic Marina M Caldon C Elizabeth CE Morton Jennifer P JP Timpson Paul P
Cell reports 20180601 11
Application of advanced intravital imaging facilitates dynamic monitoring of pathway activity upon therapeutic inhibition. Here, we assess resistance to therapeutic inhibition of the PI3K pathway within the hypoxic microenvironment of pancreatic ductal adenocarcinoma (PDAC) and identify a phenomenon whereby pronounced hypoxia-induced resistance is observed for three clinically relevant inhibitors. To address this clinical problem, we have mapped tumor hypoxia by both immunofluorescence and phosp ...[more]
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