Impact of two-bond ¹⁵N-¹⁵N scalar couplings on ¹⁵N transverse relaxation measurements for arginine side chains of proteins.

ABSTRACT: NMR relaxation of arginine (Arg) ¹⁵N_ε nuclei is useful for studying side-chain dynamics of proteins. In this work, we studied the impact of two geminal ¹⁵N-¹⁵N scalar couplings on measurements of transverse relaxation rates (R ₂ ) for Arg side-chain ¹⁵N_ε nuclei. For 12 Arg side chains of the DNA-binding domain of the Antp protein, we measured the geminal ¹⁵N-¹⁵N couplings ( ² J _NN ) of the ¹⁵N_ε nuclei and found that the magnitudes of the ² J _NN coupling constants were virtually uniform with an average of 1.2 Hz. Our simulations, assuming ideal 180° rotations for all ¹⁵N nuclei, suggested that the two ² J <

SUBMITTER: Nguyen D 

PROVIDER: S-EPMC6020141 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature