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Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes.


ABSTRACT: Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated ?-galactosidase, p21, p16, phosphorylated p38, and ?H2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

SUBMITTER: Chong Z 

PROVIDER: S-EPMC6021292 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes.

Chong Zhao Z   Matsuo Haruka H   Kuroda Mai M   Yamashita Shuntaro S   Parajuli Gopal Prasad GP   Manandhar Hira Kaji HK   Shimizu Kuniyoshi K   Katakura Yoshinori Y  

Cytotechnology 20180602 3


Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (num  ...[more]

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