Panoramic portrait of primary molecular events preceding excited state proton transfer in water.

ABSTRACT: Photochemistry powers numerous processes from luminescence and human vision, to light harvesting. However, the elucidation of multidimensional photochemical reaction coordinates on molecular timescales remains challenging. We developed wavelength-tunable femtosecond stimulated Raman spectroscopy to simultaneously achieve pre-resonance enhancement for transient reactant and product species of the widely used photoacid pyranine undergoing excited-state proton transfer (ESPT) reaction in solution. In the low-frequency region, the 280 cm^-1 ring deformation mode following 400 nm photoexcitation exhibits pronounced intensity oscillations on the sub-picosecond timescale due to anharmonic vibrational coupling to the 180 cm^-1 hydrogen-bond stretching mode only in ESPT-capable solvents, indicating a primary event of functional relevance. This leads to the contact ion pair formation on the 3 ps timescale before diffusion-controlled separation. The intermolecular 180 cm^-1 mode also reveals vibrational cooling time constants, ?500 fs and 45 ps in both H₂O and D₂O, which differ from ESPT time constants of ?3/8 and 90/250 ps in H₂O/D₂O, respectively. Spectral results using H₂¹⁸O further substantiate the functional role of the intermolecular 180 cm^-1 mode in modulating the distance between proton donor and acceptor and forming the transient ion pair. The direct observation of molecular structural evolution across a wide spectral region during photochemical reactions enriches our fundamental understanding of potential energy surface and holds the key to advancing energy and biological sciences with exceptional atomic and temporal precision.

SUBMITTER: Liu W 

PROVIDER: S-EPMC6021748 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature