Ontology highlight
ABSTRACT:
SUBMITTER: Chen L
PROVIDER: S-EPMC6022269 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Chen Liangliang L Ye Ying Y Dai Hongxia H Zhang Heyao H Zhang Xue X Wu Qiang Q Zhu Zhexin Z Spalinskas Rapolas R Ren Wenyan W Zhang Wensheng W
Stem cells international 20180614
Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors se ...[more]