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Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis.

ABSTRACT: The contribution of glutathione (GSH) in combating environmental stress in plants has long been known. Previous reports have pointed to the involvement of GSH in inducing various heat shock proteins (HSPs), but the molecular mechanism is yet to be explored. Here, we investigate how GSH induces the expression of important HSP genes in Arabidopsis. Expression of HSP genes BiP3, HSP70B, and HSP90.1 was positively regulated by GSH, and a promoter activation assay suggested a role for GSH in their induction. Lower expression of BiP3 and HSP70B in the GSH-fed Atmyb21 mutant and of HSP90.1 in the GSH-fed Atbzip10 mutant, in comparison with GSH-fed Col-0, revealed a role for GSH in activating their promoters through the transcription factors MYB21 and BZIP10. Co-transfection of transcription factor mutant protoplasts with transcription factor constructs and HSP promoters confirmed the results. Comparative proteomics also revealed proteins whose expression was controlled by MYB21 and BZIP10 in response to GSH feeding. A co-immunoprecipitation assay demonstrated a role for GSH in modulating the level of interaction of glutathione-S-transferase with HSP70. Collectively, our results demonstrate a role for GSH in activating the promoters of BiP3 and HSP70B via MYB21 and of HSP90.1 via BZIP10.

SUBMITTER: Kumar D

PROVIDER: S-EPMC6022672 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis.

Kumar Deepak D Kumar Deepak D Kumar Deepak D Kumar Deepak D Chattopadhyay Sharmila S

Journal of experimental botany 20180601 15

The contribution of glutathione (GSH) in combating environmental stress in plants has long been known. Previous reports have pointed to the involvement of GSH in inducing various heat shock proteins (HSPs), but the molecular mechanism is yet to be explored. Here, we investigate how GSH induces the expression of important HSP genes in Arabidopsis. Expression of HSP genes BiP3, HSP70B, and HSP90.1 was positively regulated by GSH, and a promoter activation assay suggested a role for GSH in their in ...[more]

PMID: 29722824

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Project description:BackgroundThe heat shock response of Arabidopsis thaliana is dependent upon a complex regulatory network involving twenty-one known transcription factors and four heat shock protein families. It is known that heat shock proteins (Hsps) and transcription factors (Hsfs) are involved in cellular response to various forms of stress besides heat. However, the role of Hsps and Hsfs under cold and non-thermal stress conditions is not well understood, and it is unclear which types of stress interact least and most strongly with Hsp and Hsf response pathways. To address this issue, we have analyzed transcriptional response profiles of Arabidopsis Hsfs and Hsps to a range of abiotic and biotic stress treatments (heat, cold, osmotic stress, salt, drought, genotoxic stress, ultraviolet light, oxidative stress, wounding, and pathogen infection) in both above and below-ground plant tissues.ResultsAll stress treatments interact with Hsf and Hsp response pathways to varying extents, suggesting considerable cross-talk between heat and non-heat stress regulatory networks. In general, Hsf and Hsp expression was strongly induced by heat, cold, salt, and osmotic stress, while other types of stress exhibited family or tissue-specific response patterns. With respect to the Hsp20 protein family, for instance, large expression responses occurred under all types of stress, with striking similarity among expression response profiles. Several genes belonging to the Hsp20, Hsp70 and Hsp100 families were specifically upregulated twelve hours after wounding in root tissue, and exhibited a parallel expression response pattern during recovery from heat stress. Among all Hsf and Hsp families, large expression responses occurred under ultraviolet-B light stress in aerial tissue (shoots) but not subterranean tissue (roots).ConclusionOur findings show that Hsf and Hsp family member genes represent an interaction point between multiple stress response pathways, and therefore warrant functional analysis under conditions apart from heat shock treatment. In addition, our analysis revealed several family and tissue-specific heat shock gene expression patterns that have not been previously described. These results have implications regarding the molecular basis of cross-tolerance in plant species, and raise new questions to be pursued in future experimental studies of the Arabidopsis heat shock response network.

Dataset Information

Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis.

Publications

Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis.

Similar Datasets

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