Unknown

Dataset Information

0

Global pairwise RNA interaction landscapes reveal core features of protein recognition.


ABSTRACT: RNA-protein interactions permeate biology. Transcription, translation, and splicing all hinge on the recognition of structured RNA elements by RNA-binding proteins. Models of RNA-protein interactions are generally limited to short linear motifs and structures because of the vast sequence sampling required to access longer elements. Here, we develop an integrated approach that calculates global pairwise interaction scores from in vitro selection and high-throughput sequencing. We examine four RNA-binding proteins of phage, viral, and human origin. Our approach reveals regulatory motifs, discriminates between regulated and non-regulated RNAs within their native genomic context, and correctly predicts the consequence of mutational events on binding activity. We design binding elements that improve binding activity in cells and infer mutational pathways that reveal permissive versus disruptive evolutionary trajectories between regulated motifs. These coupling landscapes are broadly applicable for the discovery and characterization of protein-RNA recognition at single nucleotide resolution.

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC6023938 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Global pairwise RNA interaction landscapes reveal core features of protein recognition.

Zhou Qin Q   Kunder Nikesh N   De la Paz José Alberto JA   Lasley Alexandra E AE   Bhat Vandita D VD   Morcos Faruck F   Campbell Zachary T ZT  

Nature communications 20180628 1


RNA-protein interactions permeate biology. Transcription, translation, and splicing all hinge on the recognition of structured RNA elements by RNA-binding proteins. Models of RNA-protein interactions are generally limited to short linear motifs and structures because of the vast sequence sampling required to access longer elements. Here, we develop an integrated approach that calculates global pairwise interaction scores from in vitro selection and high-throughput sequencing. We examine four RNA  ...[more]

Similar Datasets

| S-EPMC4871124 | biostudies-literature
| S-EPMC4597059 | biostudies-literature
| S-EPMC3654364 | biostudies-literature
2014-12-16 | E-GEOD-58974 | biostudies-arrayexpress
| S-EPMC4337786 | biostudies-other
2014-12-16 | GSE58974 | GEO
| S-EPMC3465099 | biostudies-literature
2021-07-23 | GSE142242 | GEO
| S-EPMC6547422 | biostudies-literature
| S-EPMC10514315 | biostudies-literature