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NeuO for Neuronal Labeling in Zebrafish.

ABSTRACT: We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.

SUBMITTER: Teoh CL

PROVIDER: S-EPMC6024405 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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NeuO for Neuronal Labeling in Zebrafish.

Teoh Chai Lean CL Er Jun Cheng JC Mukherjee Parag P Chang Young-Tae YT

Tomography (Ann Arbor, Mich.) 20150901 1

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1<i>H</i>-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-<i>s</i>-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simpl ...[more]

PMID: 30042954

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