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Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.

ABSTRACT: The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1?3) and four known (4?7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [?]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22?145 ?g/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita.

SUBMITTER: Chianese G

PROVIDER: S-EPMC6025266 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.

Chianese Giuseppina G Esposito Fortunato Palma FP Parrot Delphine D Ingham Colin C de Pascale Donatella D Tasdemir Deniz D

Marine drugs 20180528 6

The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1⁻3) and four known (4⁻7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C- ...[more]

PMID: 29843452

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Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.

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Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium <i>Aequorivita</i> sp.

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