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Microscopic study on colonization and antimicrobial property of endophytic bacteria associated with ethnomedicinal plants of Meghalaya.

ABSTRACT: Microscopic visualization using transmission electron microscopy (TEM) can provide a better understanding of endophytic colonization within ethnomedicinal plants. Bacterial endophytes were found attached to the host cell wall colonizing the aerenchyma and intercellular spaces of the epidermis and outer cortex except the vascular system. Colonization was non-uniform as single cells, doublets or in the form of microcolonies. Analysis of in vivo antibacterial action of the methanolic extracts of the isolated endophytic bacteria against Gram-positive, Streptococcus pyogenes MTCC 1925 and Gram-negative, Salmonella enterica ser. paratyphi MTCC735 pathogens has revealed the morphological damages in the tested pathogens respectively, under scanning electron microscopy (SEM). Detached cell wall and cell burst were observed in Streptococcus pyogenes where as, cell blisters were shown in Salmonella enterica ser. paratyphi. The study on bacterial endophyte colonization process is important to better predict how endophytes interact with their host and establish themselves in the plant environment by procuring biocontrol activity.

SUBMITTER: War Nongkhlaw FM

PROVIDER: S-EPMC6025718 | biostudies-literature | 2017 Jul-Sep

REPOSITORIES: biostudies-literature

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Microscopic study on colonization and antimicrobial property of endophytic bacteria associated with ethnomedicinal plants of Meghalaya.

War Nongkhlaw Fenella Mary FM Joshi S R SR

Journal of microscopy and ultrastructure 20161109 3

Microscopic visualization using transmission electron microscopy (TEM) can provide a better understanding of endophytic colonization within ethnomedicinal plants. Bacterial endophytes were found attached to the host cell wall colonizing the aerenchyma and intercellular spaces of the epidermis and outer cortex except the vascular system. Colonization was non-uniform as single cells, doublets or in the form of microcolonies. Analysis of <i>in vivo</i> antibacterial action of the methanolic extract ...[more]

PMID: 30023247

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Project description:The study aimed to evaluate the antimicrobial activity of medicinal plant extracts against the bacterial pathogens prominent in dental caries. A total of 20 plant species (herbs, shrubs and trees) belonging to 18 genera and 15 families were documented for dental caries. Antimicrobial activity of solvent extracts and essential oil from plants were determined by zone of inhibition on the growth of Streptococcus mutans (MTCC 497) and Lactobacillus acidophilus (MTCC 10307) using the agar well diffusion method. The results of in vitro antimicrobial assay prove that methanol is more successful in the extraction of phytochemicals from plant samples than aqueous solvent, as methanol extracts show higher antimicrobial activity than aqueous extracts against both the test pathogens. Methanol extracts of Nigella sativa, Psidium guajava and Syzygium aromaticum were the most effective among all 20 plant samples and have potent inhibitory activity against both dental caries pathogens with minimum inhibitory concentration of 0.2 mg mL- 1. N. sativa seed methanol extract was more effective with 22.3 mm zone of inhibition at 0.2 mg mL- 1 against S. mutans (MTCC 497), while L. acidophilus (MTCC 10307) was more sensitive to S. aromaticum bud methanol extract at 11.3 mm zone of inhibition at concentration 0.1 mg mL- 1. Essential oil extracted from plants also possesses strong antimicrobial activity for both test pathogens, with a minimum inhibitory concentration range of 0.05-0.16 mg mL- 1. Syzygium aromaticum bud essential oil at 0.05 mg mL- 1 was most active against S. mutans (MTCC 497). Plant extracts viewing antimicrobial activity with minimum inhibitory concentration show the efficacy of the plant products that could be considered as a good indicator of prospective plants for discovering new antimicrobial agents against dental caries pathogens. The findings of this study provide a lead to further polyherbal formulations for the treatment of dental caries malaise.

Project description:The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160-260-mg As kg-1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg-1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg-1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg-1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

Dataset Information

Microscopic study on colonization and antimicrobial property of endophytic bacteria associated with ethnomedicinal plants of Meghalaya.

Publications

Microscopic study on colonization and antimicrobial property of endophytic bacteria associated with ethnomedicinal plants of Meghalaya.

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