Unknown

Dataset Information

0

Development of novel EST-SSR markers for Rhododendron longipedicellatum (Ericaceae) and cross-amplification in two congeners.

ABSTRACT: Premise of the Study:To investigate the genetic background and population characteristics of Rhododendron longipedicellatum (Ericaceae), a newly discovered and critically endangered species, expressed sequence tag-simple sequence repeat markers were developed, and transferability was tested in two congeners, R. molle and R. simsii. Methods and Results:Based on the transcriptome sequences of R. longipedicellatum, 102 primer sets were designed; 48 primer sets were successfully amplified, with 15 showing polymorphisms in 150 individuals from five extant populations of R. longipedicellatum. The number of alleles per locus ranged from four to 18, and the levels of observed and expected heterozygosity for the 15 loci varied from 0.255 to 0.913 and from 0.306 to 0.851, respectively. All 15 loci were found to amplify in R. molle and R. simsii. Conclusions:These polymorphic SSR markers can be used in conservation genetic and phylogeographic studies to elucidate the rarity and origin of R. longipedicellatum.

SUBMITTER: Li T 

PROVIDER: S-EPMC6025811 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Development of novel EST-SSR markers for <i>Rhododendron longipedicellatum</i> (Ericaceae) and cross-amplification in two congeners.

Li Taiqiang T   Liu Xiongfang X   Li Zhenghong Z   Wan Youming Y   Liu Xiuxian X   Ma Hong H  

Applications in plant sciences 20180621 6

<h4>Premise of the study</h4>To investigate the genetic background and population characteristics of <i>Rhododendron longipedicellatum</i> (Ericaceae), a newly discovered and critically endangered species, expressed sequence tag-simple sequence repeat markers were developed, and transferability was tested in two congeners, <i>R. molle</i> and <i>R. simsii</i>.<h4>Methods and results</h4>Based on the transcriptome sequences of <i>R. longipedicellatum</i>, 102 primer sets were designed; 48 primer  ...[more]

Similar Datasets

Unknown Development of novel EST-SSR markers for Phyllanthus emblica (Phyllanthaceae) and cross-amplification in two related species.
| S-EPMC6055576 | biostudies-literature
Unknown Development of EST-SSR markers in Barringtonia racemosa (Lecythidaceae) and cross-amplification in related species.
| S-EPMC4683042 | biostudies-literature
Unknown Development of polymorphic EST-SSR markers in Itea chinensis (Iteaceae) and cross-amplification in related species.
| S-EPMC5828125 | biostudies-literature
Unknown Development of EST-SSR markers for <i>Pluchea indica</i> (Asteraceae) and cross-amplification in related species.
| S-EPMC6110239 | biostudies-literature
Unknown Development of EST-SSR markers in <i>Saxifraga sinomontana</i> (Saxifragaceae) and cross-amplification in three related species.
| S-EPMC6580987 | biostudies-literature
Unknown Development of EST-SSR markers for <i>Pongamia pinnata</i> by transcriptome database mining: cross-species amplification and genetic diversity.
| S-EPMC7688882 | biostudies-literature
Unknown Development of 16 novel EST-SSR markers for species identification and cross-genus amplification in sambar, sika, and red deer.
| S-EPMC8975116 | biostudies-literature
Unknown Development of EST-SSR markers of Ipomoea nil.
| S-EPMC3405949 | biostudies-literature
Unknown Isolation and characterization of 46 novel polymorphic EST-simple sequence repeats (SSR) markers in two Sinipercine Fishes (Siniperca) and cross-species amplification.
| S-EPMC3431812 | biostudies-literature
Unknown Isolation and characterization of novel genomic and EST-SSR markers in Coreoperca whiteheadi Boulenger and cross-species amplification.
| S-EPMC3497321 | biostudies-literature