Dataset Information

Identification and characterization of microsatellites in Aconitum reclinatum (Ranunculaceae), a rare species endemic to North America.

ABSTRACT: Premise of the Study:Aconitum reclinatum is the only representative species of Aconitum subg. Lycoctonum in North America, with restricted ranges and endangered populations. Polymorphic microsatellite markers were developed for A. reclinatum for further investigation of genetic diversity and population structure. Methods and Results:Using Illumina HiSeq technology, we sequenced a genomic library for identification of simple sequence repeat markers. A total of 12 polymorphic primer pairs were developed and tested on 66 individuals from four populations in North America. The number of alleles ranged from one to seven per locus with an average of 3.48. Levels of observed and expected heterozygosity varied from 0 to 1.000 and 0 to 0.736, respectively, at population level. Three primer pairs were successfully amplified in three of four closely related species. Conclusions:The microsatellites isolated in this study will be useful in further research on the genetic diversity and conservation genetics of A. reclinatum populations in North America.

SUBMITTER: Zhou J

PROVIDER: S-EPMC6025813 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Identification and characterization of microsatellites in <i>Aconitum reclinatum</i> (Ranunculaceae), a rare species endemic to North America.

Zhou Juan J Liu Wanzhen W Kong Hanghui H Gong Wei W

Applications in plant sciences 20180624 6

<h4>Premise of the study</h4><i>Aconitum reclinatum</i> is the only representative species of <i>Aconitum</i> subg. <i>Lycoctonum</i> in North America, with restricted ranges and endangered populations. Polymorphic microsatellite markers were developed for <i>A. reclinatum</i> for further investigation of genetic diversity and population structure.<h4>Methods and results</h4>Using Illumina HiSeq technology, we sequenced a genomic library for identification of simple sequence repeat markers. A to ...[more]

PMID: 30131903

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Project description:Worldwide, the genus Ranunculus includes approximately 600 species and is highly genetically diverse. Recent taxonomic reports suggest that the genus has a monophyletic origin, divided into two subgenera, and consists of 17 sections. The Central Asian country of Kazakhstan has 62 species of the genus that have primarily been collected in the central part of the country. The latest collection trips in southern parts of the country have led to the description of a wider distribution area for Ranunculus and the identification of a new species Ranunculus talassicus Schegol. et A.L. Ebel from Western Tien Shan. Therefore, in this study, attempts were made to assess the molecular taxonomic positions of R. talassicus and two other species endemic to the Central Asian region R. karkaralensis Schegol. and R. pskemensis V.N. Pavlov in relation to other species of the genus, using internal transcribed spacer (ITS) molecular genetic markers. The ITS-aligned sequences of 22 local Central Asian accessions and 43 accession sequences available in the National Center for Biotechnology Information (NCBI) database allowed the construction of a maximum parsimony phylogenetic tree and a Neighbor-Net network. The results indicated that R. talassicus and R. pskemensis could be assigned to section Ranunculastrum. Additionally, an assessment of the network suggested that R. pskemensis was the rooting taxon for the group of species containing R. talassicus, and that R. illyricus L. and R. pedatus Waldst. & Kit. were founders of a prime rooting node for the Ranunculastrum section of the genus. The ITS-aligned sequences showed that R. karkaralensis was indifferent with respect to three other species in the Ranunculus section of the genus, i.e., R. acris L., R. grandifolius C.A. Mey., and R. subborealis Tzvelev. The study indicated that the assessments of ITS-based phylogenetic tree and Neighbor-Net network provided new insights into the taxonomic positions of three endemic species from Central Asia.

Dataset Information

Identification and characterization of microsatellites in Aconitum reclinatum (Ranunculaceae), a rare species endemic to North America.

Publications

Identification and characterization of microsatellites in <i>Aconitum reclinatum</i> (Ranunculaceae), a rare species endemic to North America.

Similar Datasets

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