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Revealing Compartmentalized Diffusion in Living Cells with Interferometric Scattering Microscopy.

ABSTRACT: The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background suppression, at the expense of imaging speed and observation time. Here, we show that interferometric scattering microscopy combined with 40 nm gold nanoparticle labeling can be used to follow the motion of membrane proteins in the plasma membrane of live cultured mammalian cell lines and hippocampal neurons with up to 3 nm precision and 25 ?s temporal resolution. The achievable spatiotemporal precision enabled us to reveal signatures of compartmentalization in neurons likely caused by the actin cytoskeleton.

SUBMITTER: de Wit G

PROVIDER: S-EPMC6026387 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Revealing Compartmentalized Diffusion in Living Cells with Interferometric Scattering Microscopy.

de Wit Gabrielle G Albrecht David D Ewers Helge H Kukura Philipp P

Biophysical journal 20180601 12

The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background suppression, at the expense of imaging speed and observation time. Here, we show that interferometric scattering microscopy combined with 40 nm gold nanoparticle labeling can be used ...[more]

PMID: 29925030

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