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The Quorum Sensing System of Yersinia enterocolitica 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance.


ABSTRACT: Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene, ycoR, that is not linked to an AHL synthase. To explore the contribution of yenR/yenI/ycoR to QS-dependent phenotypes in Yersinia enterocolitica strain 8081, single and multiple mutants were constructed. AHL profiling identified N-(3-oxohexanoyl) homoserine lactone, N-hexanoylhomoserine lactone, and N-(3-oxoseptanoyl) homoserine lactone as the most abundant. The AHL profiles of the yenR, ycoR and yenR/ycoR mutants were similar to the parent suggesting that the two LuxR homologues do not regulate AHL production while the yenI mutants were AHL-negative. A role for QS in swimming motility and cell attachment was demonstrated. Down-regulation of the virulence plasmid partition gene, spyA, in yenI and yenI/yenR/ycoR mutants is consistent with the greater loss of the Y. enterocolitica pYVe virulence plasmid in the yenI mutant during serial passage at 37 °C but not at 22 °C. A role for QS-regulated spyA in virulence plasmid maintenance is suggested.

SUBMITTER: Ng YK 

PROVIDER: S-EPMC6027161 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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The Quorum Sensing System of <i>Yersinia enterocolitica</i> 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance.

Ng Yen-Kuan YK   Grasso Marco M   Wright Victoria V   Garcia Vanina V   Williams Paul P   Atkinson Steve S  

Genes 20180620 6


Although <i>Yersinia enterocolitica</i> genomes are highly heterogeneous, they contain a conserved <i>N</i>-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the <i>luxR</i> and <i>luxI</i> orthologs <i>yenR</i> and <i>yenI</i> respectively. Certain hypervirulent strains also contain a putative orphan <i>luxR</i> gene, <i>ycoR</i>, that is not linked to an AHL synthase. To explore the contribution of <i>yenR/yenI/ycoR</i> to QS-dependent phenotypes in <i>Yersinia en  ...[more]

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