Dataset Information

DNA Barcoding to Confirm the Morphological Identification of the Coral Trees (Erythrina spp., Fabaceae) in the Ancient Gardens of Naples (Campania, Italy).

ABSTRACT: The coral trees (genus Erythrina) have been fostering great interest among the botanists and gardeners of Naples, since their arrival in Europe in the second half of the 18th century. Numerous species were present in the royal and private botanical gardens of the region, but their number has decreased today. The purpose of this work was to verify which species occur nowadays in the public areas of Naples and associate them with the historical information about their introduction. The identification was carried out also by molecular methods, by means of sequencing nuclear and chloroplast DNA markers. The comparison of the sequences obtained for the specimens present in Naples with those present in the literature, together with a morphological examination, allowed us to identify with accuracy the species anciently introduced or nowadays cultivated in Naples.

SUBMITTER: De Luca A

PROVIDER: S-EPMC6027229 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

DNA Barcoding to Confirm the Morphological Identification of the Coral Trees (<i>Erythrina</i> spp., Fabaceae) in the Ancient Gardens of Naples (Campania, Italy).

De Luca Adriana A Sibilio Giancarlo G De Luca Paolo P Del Guacchio Emanuele E

Plants (Basel, Switzerland) 20180606 2

The coral trees (genus <i>Erythrina</i>) have been fostering great interest among the botanists and gardeners of Naples, since their arrival in Europe in the second half of the 18th century. Numerous species were present in the royal and private botanical gardens of the region, but their number has decreased today. The purpose of this work was to verify which species occur nowadays in the public areas of Naples and associate them with the historical information about their introduction. The iden ...[more]

PMID: 29882805

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Project description:BackgroundDNA barcoding have been considered as a tool to facilitate species identification based on its simplicity and high-level accuracy in compression to the complexity and subjective biases linked to morphological identification of taxa. MaturaseK gene (MatK gene) of the chloroplast is very vital in the plant system which is involved in the group II intron splicing. The main objective of this study is to determine the relative utility of the "MatK" chloroplast gene for barcoding in 15 legume as a tool to facilitate species identification based on their simplicity and high-level accuracy linked to morphological identification of taxa.Methods and resultsMatK gene sequences were submitted to GenBank and the accession numbers were obtained with sequence length ranging from 730 to 1545 nucleotides. These DNA sequences were aligned with database sequence using PROMALS server, Clustal Omega server and Bioedit program. Maximum likelihood and neighbor-joining algorithms were employed for constructing phylogeny. Overall, these results indicated that the phylogenetic tree analysis and the evolutionary distances of an individual dataset of each species were agreed with a phylogenetic tree of all each other consisting of two clades, the first clade comprising (Enterolobium contortisiliquum, Albizia lebbek), Acacia saligna, Leucaena leucocephala, Dichrostachys Cinerea, (Delonix regia, Parkinsonia aculeata), (Senna surattensis, Cassia fistula, Cassia javanica) and Schotia brachypetala were more closely to each other, respectively. The remaining four species of Erythrina humeana, (Sophora secundiflora, Dalbergia Sissoo, Tipuana Tipu) constituted the second clade.ConclusionMoreover, their sequences could be successfully utilized in single nucleotide polymorphism or as part of the sequence as DNA fragment analysis utilizing polymerase chain reaction in plant systematic. Therefore, MatK gene is considered promising a candidate for DNA barcoding in the plant family Fabaceae and provides a clear relationship between the families.

Project description:Prenylated (iso)flavonoids, -flavans and pterocarpans from taxa in Erythrina are repeatedly flagged as potent antimicrobial compounds. In the current study, bark from E. lysistemon was extracted and seven isoflavone derivatives were purified: erybraedin A (1), phaseollidin (2), abyssinone V-4' methyl ether (3), eryzerin C (4), alpumisoflavone (5), cristacarpin (6) and lysisteisoflavone (7). Minimum inhibition concentration (MIC) values were determined against a range of species of bacteria (skin pathogens), then values for another 67 derivatives from Erythrina, only against Staphylococcus aureus, were mined from the literature. Of the seven isolates, MIC values widely ranged from 1-600 ?g/mL, with no obvious pattern of selectivity for Gram-types. Nevertheless, using the mined and experimentally determined values against S. aureus, Klekota-Roth fragments (Structure Activity Relationship: SAR) were determined then used as molecular descriptors to make a 'decision tree' based on structural characters inspired by the classes of antimicrobial potency (classes A-D). Furthermore, to make quantitative predictions of MIC values (Quantitative SAR: QSAR) 'pace regression' was utilized and validated (R² = 0.778, Q² = 0.727 and P² = 0.555). Evidently, the position and degree of prenylation is important; however, the presence of hydroxyl groups at positions 5 and 7 in ring A and 4' in ring B is associated with lower MIC values. While antimicrobial results continue to validate the traditional use of E. lysistemon extracts (or Erythrina generally) in therapeutic applications consistent with anti-infection, it is surprising that this class of compound is not being utilized more often in general industry applications, such as food or cosmetic preservation, or in topical antimicrobial creams. Prenylated (iso)flavonoids are derived from several other Genera, such as Dorstenia (Moraceae), Ficus (Moraceae), Glycyrrhiza (Fabaceae), Paulownia (Lamiales) or Pomifera (Moraceae).

Dataset Information

DNA Barcoding to Confirm the Morphological Identification of the Coral Trees (Erythrina spp., Fabaceae) in the Ancient Gardens of Naples (Campania, Italy).

Publications

DNA Barcoding to Confirm the Morphological Identification of the Coral Trees (<i>Erythrina</i> spp., Fabaceae) in the Ancient Gardens of Naples (Campania, Italy).

Similar Datasets

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