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The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages.

ABSTRACT: We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201?2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we investigate the replication pathway of the Pseudomonas aeruginosa bacteriophages ?KZ and ?PA3. Bacteriophages ?KZ and ?PA3 encode a proteinaceous shell that assembles a nucleus-like structure that compartmentalizes proteins and DNA during viral infection. We show that the tubulin-like protein PhuZ encoded by each phage assembles a bipolar spindle that displays dynamic instability and positions the nucleus at midcell. Our results suggest that the phage spindle and nucleus play the same functional role in all three phages, 201?2-1, ?KZ, and ?PA3, demonstrating that these key structures are conserved among large Pseudomonas phages.

SUBMITTER: Chaikeeratisak V

PROVIDER: S-EPMC6028189 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages.

Chaikeeratisak Vorrapon V Nguyen Katrina K Egan MacKennon E ME Erb Marcella L ML Vavilina Anastasia A Pogliano Joe J

Cell reports 20170801 7

We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201φ2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we investigate the replication pathway of the Pseudomonas aeruginosa bacteriophages φKZ and φPA3. Bacteriophages φKZ and φPA3 encode a proteinaceous shell that assembles a nucleus-like structure that compartm ...[more]

PMID: 28813669

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