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Investigation of function and regulation of the YB-1 cellular factor in HIV replication.

ABSTRACT: Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions. [BMB Reports 2018; 51(6): 290-295].

SUBMITTER: Jung YM

PROVIDER: S-EPMC6033064 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Investigation of function and regulation of the YB-1 cellular factor in HIV replication.

Jung Yu-Mi YM Yu Kyung-Lee KL Park Seong-Hyun SH Lee Seong-Deok SD Kim Min-Jeong MJ You Ji-Chang JC

BMB reports 20180601 6

Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 ...[more]

PMID: 29429449

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