Unknown

Dataset Information

0

Detection of plant protein adulterated in fluid milk using two-dimensional gel electrophoresis combined with mass spectrometry.


ABSTRACT: The illegal or unlabelled addition of plant protein in milk can cause serious anaphylaxis. For sustainable food security, it is therefore important to develop a methodology to detect non-milk protein in milk products. This research aims to differentiate milk adulterated with plant protein using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. According to the protein spots highlighted on the gel of adulterated milk, ?-conglycinin and glycinin were detected in milk adulterated with soy protein, while legumin, vicilin, and convicilin indicated the addition of pea protein, and ?-amylase and serpin marked wheat protein. These results suggest that a 2-DE-based protein profile is a useful method to identify milk adulterated with soy and pea protein, with a detection limit of 4% plant protein in the total protein.

SUBMITTER: Yang J 

PROVIDER: S-EPMC6033788 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Detection of plant protein adulterated in fluid milk using two-dimensional gel electrophoresis combined with mass spectrometry.

Yang Jinhui J   Zheng Nan N   Yang Yongxin Y   Wang Jiaqi J   Soyeurt Hélène H  

Journal of food science and technology 20180507 7


The illegal or unlabelled addition of plant protein in milk can cause serious anaphylaxis. For sustainable food security, it is therefore important to develop a methodology to detect non-milk protein in milk products. This research aims to differentiate milk adulterated with plant protein using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry. According to the protein spots highlighted on the gel of adulterated milk, β-conglycinin and glycinin were detected in milk adult  ...[more]

Similar Datasets

| S-EPMC2650687 | biostudies-literature
| S-EPMC3206423 | biostudies-literature
| S-EPMC535397 | biostudies-literature
| S-EPMC6121189 | biostudies-literature
| S-EPMC7694097 | biostudies-literature
| S-EPMC317362 | biostudies-literature
| S-EPMC1282590 | biostudies-literature
| S-EPMC3277759 | biostudies-literature
| S-EPMC4016294 | biostudies-literature
| S-EPMC5596216 | biostudies-literature