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Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria.

ABSTRACT: The intestine is a highly complex ecosystem where many bacterial species interact with each other and host cells to influence animal physiology and susceptibility to pathogens. Genomic methods have provided a broad framework for understanding how alterations in microbial communities are associated with host physiology and infection, but the biochemical mechanisms of specific intestinal bacterial species are only emerging. In this review, we focus on recent studies that have characterized the biochemical mechanisms by which intestinal bacteria interact with other bacteria and host pathways to restrict pathogen infection. Understanding the biochemical mechanisms of intestinal microbiota function should provide new opportunities for therapeutic development towards a variety of infectious diseases.

SUBMITTER: Rangan KJ

PROVIDER: S-EPMC6038137 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria.

Rangan Kavita J KJ Hang Howard C HC

Trends in biochemical sciences 20170917 11

The intestine is a highly complex ecosystem where many bacterial species interact with each other and host cells to influence animal physiology and susceptibility to pathogens. Genomic methods have provided a broad framework for understanding how alterations in microbial communities are associated with host physiology and infection, but the biochemical mechanisms of specific intestinal bacterial species are only emerging. In this review, we focus on recent studies that have characterized the bio ...[more]

PMID: 28927699

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Project description:Temperature plays an important role in bacteria-host interactions and can be a determining factor for host switching. In this study we sought to investigate the reasons behind growth temperature restriction in the entomopathogenic enterobacterium Photorhabdus. Photorhabdus has a complex dual symbiotic and pathogenic life cycle. The genus consists of 19 species but only one subgroup, previously all classed together as Photorhabdus asymbiotica, have been shown to cause human disease. These clinical isolates necessarily need to be able to grow at 37°C, whilst the remaining species are largely restricted to growth temperatures below 34°C and are therefore unable to infect mammalian hosts. Here, we have isolated spontaneous mutant lines of Photorhabdus laumondii DJC that were able to grow up to 36-37°C. Following whole genome sequencing of 29 of these mutants we identified a single gene, encoding a protein with a RecG-like helicase domain that for the majority of isolates contained single nucleotide polymorphisms. Importantly, provision of the wild-type allele of this gene in trans restored the temperature restriction, confirming the mutations are recessive, and the dominant effect of the protein product of this gene. The gene appears to be part of a short three cistron operon, which we have termed the Temperature Restricting Locus (TRL). Transcription reporter strains revealed that this operon is induced upon the switch from 30 to 36°C, leading to replication arrest of the bacteria. TRL is absent from all of the human pathogenic species so far examined, although its presence is not uniform in different strains of the Photorhabdus luminescens subgroup. In a wider context, the presence of this gene is not limited to Photorhabdus, being found in phylogenetically diverse proteobacteria. We therefore suggest that this system may play a more fundamental role in temperature restriction in diverse species, relating to as yet cryptic aspects of their ecological niches and life cycle requirements.

Project description:Salinity is one of the most severe abiotic stress in the world. Also, the irrigated lands have been treated with second salinity. Canola is one of the most important industrial crops for oil production all over the world which is affected by salinity. Salt stress causes imbalanced ion hemostasis (Na+ and K+) and interrupted mineral absorption in canola. Also, salinity stress leads to oxidative stress (production and accumulation of reactive oxygen species (ROS). Accumulation of ROS is extremely dangerous and lethal for plants. As a consequence, canola production is reduced under salinity stress. So, a suitable approach should be found to deal with salinity stress and prevent the loss of production oilseed. Plant growth-promoting rhizobacteria (PGPR) can colonize on the plant root surface and alleviate the salt stress effect by providing minerals like nitrogen, phosphate, and potassium. Also, they alleviate salt stress by phytohormones like auxin (IAA), cytokinin (CK), and abscisic acid (ABA). This study focus on physiological parameters like leaf area (LA), root length (RL), shoot length (SL), chlorophyll fluorescence indexes (Fv/Fm and Fv/F0), relative water content (RWC), electrolyte leakage index (ELI), photosynthesis pigments (chlorophyll a, b, and carotenoids), Na+, and K+; and biochemical parameters like malondialdehyde (MDA) content, hydrogen peroxide content (H2O2), total protein content, proline, antioxidant capacity, and antioxidant enzyme activities in canola through the inoculation with Enterobacter sp. S16-3 and Pseudomonas sp. C16-2O. This study showed that LA, RL, SL, chlorophyll fluorescence indexes, RWC were significantly increased and ELI was significantly decreased in bacteria inoculated treatments. Also, MDA, H2O2 were decreased, and antioxidant capacity, proline, and antioxidant enzymes were increased due to inoculation with these bacteria. Besides, the amount of K+ as an index of salinity tolerance significantly increased, and leaf Na+ content was significantly decreased.

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Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria.

Publications

Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria.

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