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Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.


ABSTRACT: OBJECTIVE:We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area. RESULTS:The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.

SUBMITTER: Senra C 

PROVIDER: S-EPMC6042422 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA.

Senra Carolina C   Gomes Luciana Inácia LI   Siqueira Liliane Maria Vidal LMV   Coelho Paulo Marcos Zech PMZ   Rabello Ana A   Oliveira Edward E  

BMC research notes 20180711 1


<h4>Objective</h4>We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area.<h4>Results</h4>The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.  ...[more]

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