Exploring the Scope of Photo-Induced Electron Transfer-Chelation-Enhanced Fluorescence-Fluorescence Resonance Energy Transfer Processes for Recognition and Discrimination of Zn²⁺, Cd²⁺, Hg²⁺, and Al³⁺ in a Ratiometric Manner: Application to Sea Fish Analysis.

ABSTRACT: A rhodamine-based smart probe (RHES) has been developed for trace-level detection and discrimination of multiple cations, viz. Al³⁺, Zn²⁺, Cd²⁺, and Hg²⁺ in a ratiometric manner involving photo-induced electron transfer-chelation-enhanced fluorescence-fluorescence resonance energy transfer processes. The method being very fast and highly selective allows their bare eye visualization at a physiological pH. The optimized geometry and spectral properties of RHES and its cation adducts have been analyzed by time-dependent density functional theory calculations. RHES detects as low as 1.5 × 10^-9 M Al³⁺, 1.2 × 10^-9 M Zn²⁺, 6.7 × 10^-9 M Cd²⁺, and 1.7 × 10^-10 M Hg²⁺, whereas the respective association constants are 1.33 × 10⁵ M^-1, 2.11 × 10⁴ M^-1, 1.35 × 10⁵ M^-1, and 4.09 × 10⁵ M^-1. The other common ions do not interfere. The probe is useful for intracellular imaging of Zn²⁺, Cd²⁺, and Hg²⁺ in squamous epithelial cells. RHES is useful for the determination of the ions in sea fish and real samples.

SUBMITTER: Ghosh M 

PROVIDER: S-EPMC6044824 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature