Potentiometric Adsorption Isotherm Analysis of a Molecularly Imprinted Polymer Interface for Small-Biomolecule Recognition.

ABSTRACT: In this paper, we report a direct and quantitative analytical method of small-biomolecule recognition with a molecularly imprinted polymer (MIP) interface, taking advantage of the potentiometric principle of a field-effect transistor (FET) sensor, which enables the direct detection of ionic charges without using labeling materials such as fluorescent dyes. The interaction of low-molecular-weight oligosaccharides such as paromomycin and kanamycin with the MIP interface including phenylboronic acid (PBA) was directly and quantitatively analyzed from the electrical signals of an MIP-coated FET sensor. In particular, the change in the potential response of the FET sensor was derived on the basis of the multi-Langmuir adsorption isotherm equations, considering the change in the molecular charge

SUBMITTER: Nishitani S 

PROVIDER: S-EPMC6045357 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature