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Potentiometric Adsorption Isotherm Analysis of a Molecularly Imprinted Polymer Interface for Small-Biomolecule Recognition.

ABSTRACT: In this paper, we report a direct and quantitative analytical method of small-biomolecule recognition with a molecularly imprinted polymer (MIP) interface, taking advantage of the potentiometric principle of a field-effect transistor (FET) sensor, which enables the direct detection of ionic charges without using labeling materials such as fluorescent dyes. The interaction of low-molecular-weight oligosaccharides such as paromomycin and kanamycin with the MIP interface including phenylboronic acid (PBA) was directly and quantitatively analyzed from the electrical signals of an MIP-coated FET sensor. In particular, the change in the potential response of the FET sensor was derived on the basis of the multi-Langmuir adsorption isotherm equations, considering the change in the molecular charges of PBA caused by the adsorption equilibrium of the analytes with the vinyl PBA-copolymerized MIP membrane. Thus, the potentiometric adsorption isotherm analysis can elucidate the formation of selective binding sites at the MIP interface. The electrochemical analysis of the functional biointerface used in this study supports the design and construction of sensors for small biomarkers.

SUBMITTER: Nishitani S

PROVIDER: S-EPMC6045357 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Polymer Composition Primarily Determines the Protein Recognition Characteristics of Molecularly Imprinted Hydrogels.

Project description:Synthetic hydrogels with the ability to recognize and bind target proteins are useful for a number of applications, including biosensing and therapeutic agent delivery. One popular method for fabricating recognitive hydrogels is molecular imprinting. A long-standing hypothesis of the field is that these molecularly imprinted polymers (MIPs) retain the chemical and geometric profile of their protein template, resulting in subsequent ability to recognize the template in solution. Here, we systematically determined the influence of network composition, as well as the identity, amount, and extraction of imprinting templates, on the protein binding of MIPs. Network composition (i.e. the relative number of ionizable and hydrophobic groups) explained the extent of protein adsorption in all cases. The identity and amount of imprinting template, albeit a protein or synthetic polymer (PEG) of similar molecular weight, did not significantly influence the amount of protein bound. While the purification method influenced the extent of template adsorption, it did so by chemically modifying the network (acrylamide hydrolysis, increasing the acid content by up to 21%) and not by voiding occupied MIP pores. Therefore, our results indicate that material composition determines the extent to which MIPs bind template and non-template proteins.

| S-EPMC7807727 | biostudies-literature

Molecularly Imprinted Polymer Materials as Selective Recognition Sorbents for Explosives: A Review.

Project description:Explosives are of significant interest to homeland security departments and forensic investigations. Fast, sensitive and selective detection of these chemicals is of great concern for security purposes as well as for triage and decontamination in contaminated areas. To this end, selective sorbents with fast binding kinetics and high binding capacity, either in combination with a sensor transducer or a sampling/sample-preparation method, are required. Molecularly imprinted polymers (MIPs) show promise as cost-effective and rugged artificial selective sorbents, which have a wide variety of applications. This manuscript reviews the innovative strategies developed in 57 manuscripts (published from 2006 to 2019) to use MIP materials for explosives. To the best of our knowledge, there are currently no commercially available MIP-modified sensors or sample preparation methods for explosives in the market. We believe that this review provides information to give insight into the future prospects and potential commercialization of such materials. We warn the readers of the hazards of working with explosives.

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| S-EPMC7547379 | biostudies-literature

Preparation, Characterization and Application of a Molecularly Imprinted Polymer for Selective Recognition of Sulpiride.

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| S-EPMC5459076 | biostudies-other

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| S-EPMC8402217 | biostudies-literature

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