Thrombin and Plasmin Alter the Proteome of Neutrophil Extracellular Traps.

ABSTRACT: Neutrophil extracellular traps (NETs) consist of a decondensed DNA scaffold decorated with neutrophil-derived proteins. The proteome of NETs, or "NETome," has been largely elucidated in vitro. However, components such as plasma and extracellular matrix proteins may affect the NETome under physiological conditions. Here, using a reductionistic approach, we explored the effects of two proteases active during injury and wounding, human thrombin and plasmin, on the NETome. Using high-resolution mass spectrometry, we identified a total of 164 proteins, including those previously not described in NETs. The serine proteases, particularly thrombin, were also found to interact with DNA and bound to NETs in vitro. Among the most abundant proteins were those identified previously, inclu

SUBMITTER: Lim CH 

PROVIDER: S-EPMC6046383 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature