Ontology highlight
ABSTRACT:
SUBMITTER: Kawakami R
PROVIDER: S-EPMC6047364 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Kawakami Ryushi R Ohshida Tatsuya T Sakuraba Haruhiko H Ohshima Toshihisa T
Frontiers in microbiology 20180709
We recently identified and characterized a novel broad substrate specificity amino acid racemase (BAR) from the hyperthermophilic archaeon <i>Pyrococcus horikoshii</i> OT-3. Three genes, <i>PH0782</i>, <i>PH1423</i>, and <i>PH1501</i>, encoding homologs exhibiting about 45% sequence identity with BAR were present in the <i>P. horikoshii</i> genome. In this study, we detected pyridoxal 5'-phosphate (PLP)-dependent amino acid racemase activity in the protein encoded by <i>PH0782</i>. The enzyme sh ...[more]