Intestinal Lymphatic Endothelial Cells Produce R-Spondin3.

ABSTRACT: The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45^-CD90⁺CD31⁺ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD)

SUBMITTER: Ogasawara R 

PROVIDER: S-EPMC6048029 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature