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A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression.


ABSTRACT: The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced a novel platform, PanelChip™ Analysis System, which provides a sensitive solution for the analysis of miRNA levels in blood. After conducting miRQC analysis, the system's analytical performance compared favorably against similar nanoscale qPCR-based array technologies. Because PanelChip™ requires only a minimal amount of miRNA for analysis, we used it to screen for potential diagnostic biomarkers in the plasma of patients with oral cavity squamous cell carcinoma (OSCC). Combining the platform with a machine learning algorithm, we were able to discover miRNA expression patterns capable of separating healthy subjects from patients with OSCC.

SUBMITTER: Hsieh CH 

PROVIDER: S-EPMC6048151 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression.

Hsieh Chia-Hsun CH   Chen Wei-Ming WM   Hsieh Yi-Shan YS   Fan Ya-Chun YC   Yang Pok Eric PE   Kang Shih-Ting ST   Liao Chun-Ta CT  

Scientific reports 20180716 1


The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced a novel platform, PanelChip™ Analysis System, which provides a sensitive solution for the analysis of miRNA levels in blood. After conducting miRQC analysis, the system's analytical performance compared favora  ...[more]

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