Dataset Information

CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.

ABSTRACT: Macrophages and their monocyte precursors mediate innate immune responses and can promote a spectrum of phenotypes from pro-inflammatory to pro-resolving. Currently, there are few markers that allow for robust dissection of macrophage phenotype. We recently identified CD38 as a marker of inflammatory macrophages in murine in vitro and in vivo models. However, it is unknown whether CD38 plays a similar marker and/or functional role in human macrophages and inflammatory diseases. Here, we establish that CD38 transcript and protein are robustly induced in human macrophages exposed to LPS (±IFN-?) inflammatory stimuli, but not with the alternative stimulus, IL-4. Pharmacologic and/or genetic CD38 loss-of-function significantly reduced the secretion of inflammatory cytokines IL-6 and IL-12p40 and glycolytic activity in human primary macrophages. Finally, monocyte analyses in systemic lupus erythematosus patients revealed that, while all monocytes express CD38, high CD38 expression in the non-classical monocyte subpopulation is associated with disease. These data are consistent with an inflammatory marker role for CD38 in human macrophages and monocytes.

SUBMITTER: Amici SA

PROVIDER: S-EPMC6048227 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.

Amici Stephanie A SA Young Nicholas A NA Narvaez-Miranda Janiret J Jablonski Kyle A KA Arcos Jesus J Rosas Lucia L Papenfuss Tracey L TL Torrelles Jordi B JB Jarjour Wael N WN Guerau-de-Arellano Mireia M

Frontiers in immunology 20180710

Macrophages and their monocyte precursors mediate innate immune responses and can promote a spectrum of phenotypes from pro-inflammatory to pro-resolving. Currently, there are few markers that allow for robust dissection of macrophage phenotype. We recently identified CD38 as a marker of inflammatory macrophages in murine <i>in vitro</i> and <i>in vivo</i> models. However, it is unknown whether CD38 plays a similar marker and/or functional role in human macrophages and inflammatory diseases. Her ...[more]

PMID: 30042766

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Dataset Information

CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.

Publications

CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.

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