Ontology highlight
ABSTRACT:
SUBMITTER: Ward A
PROVIDER: S-EPMC6048317 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Ward Alyssa A Kumari Gita G Sen Ranjan R Desiderio Stephen S
Molecular and cellular biology 20180716 15
Accessibility of antigen receptor loci to RAG is correlated with the presence of H3K4me3, which binds to a plant homeodomain (PHD) in the RAG-2 subunit and promotes V(D)J recombination. A point mutation in the PHD, W453A, eliminates binding of H3K4me3 and impairs recombination. The debilitating effect of the W453A mutation is ameliorated by second-site mutations that locate an inhibitory domain in the interval from residues 352 through 405 of RAG-2. Disruption of the inhibitory domain stimulates ...[more]