Dataset Information

Molecular cloning and functional analysis of the promoter of ?-Tocopherol Methyl Transferase (?-TMT) gene of soybean (Glycine max).

ABSTRACT: ?-Tocopherol methyl transferase (?-TMT) (EC 2.1.1.95) is the key enzyme of the tocopherol biosynthetic pathway that determines the ?-tocopherol concentration in plants. The overexpression of ?-TMT has been a successful approach for ?-tocopherol enrichment of most plants including soybean. The typical soybean varieties are rich in ?-tocopherol (constitutes nearly 65-70% of its total seed tocopherol pool), while ?-tocopherol, the biologically most active form among all tocopherols, constitutes only 10% of the total tocopherol content. The identification of soybean varieties that have seed ?-tocopherol as high as >?20% of the total tocopherols has shifted attention towards the breeding based approach for ?-tocopherol enrichment of this crop. Previous research on this aspect suggests that polymorphisms in ?-TMT promoter might be associated with the high ?-tocopherol concentration of some soybean varieties. To understand the molecular basis of genetic variation for ?-tocopherol concentration in Indian varieties of soybean we cloned the 1.4 kb upstream promoter region of ?-TMT from a high ?-tocopherol containing soybean variety (Bragg) as well as from a low ?-tocopherol containing variety (DS 2706). Cloning of each of these promoters in pORE R2 vector having GUS reporter gene and the subsequent GUS assay revealed a slightly high promoter activity of Bragg ?-TMT as compared to DS 2706 ?-TMT. On promoter sequence analysis, no sequence polymorphisms were observed in the core promoter region of this gene. However, seven single nucleotide polymorphisms (SNPs) were observed outside the core promoter region. Further study based on deletion construct analysis of this promoter will elucidate the significance of these SNPs in influencing the activity of ?-TMT promoter and the ?-tocopherol concentration.

SUBMITTER: Tewari K

PROVIDER: S-EPMC6050176 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular cloning and functional analysis of the promoter of γ-Tocopherol Methyl Transferase (γ-TMT) gene of soybean (Glycine max).

Tewari Kalpana K Kumar Vaibhav V Kumar Amresh A Bansal Navita N Vinutha T T Ali Kishwar K Sachdev Archana A Kumari Sweta S Dahuja Anil A

3 Biotech 20180717 8

γ-Tocopherol methyl transferase (γ-TMT) (EC 2.1.1.95) is the key enzyme of the tocopherol biosynthetic pathway that determines the α-tocopherol concentration in plants. The overexpression of γ-TMT has been a successful approach for α-tocopherol enrichment of most plants including soybean. The typical soybean varieties are rich in γ-tocopherol (constitutes nearly 65-70% of its total seed tocopherol pool), while α-tocopherol, the biologically most active form among all tocopherols, constitu ...[more]

PMID: 30034989

Similar Datasets

Project description:Soybean (Glycine max) is an important leguminous crop that is grown throughout the United States and around the world. In 2016, soybean was valued at $41 billion USD in the United States alone. Increasingly, soybean farmers are adopting alternative management strategies to improve the sustainability and profitability of their crop. Various benefits have been demonstrated for alternative management systems, but their effects on soybean-associated microbial communities are not well-understood. In order to better understand the impact of crop management systems on the soybean-associated microbiome, we employed DNA amplicon sequencing of the Internal Transcribed Spacer (ITS) region and 16S rRNA genes to analyze fungal and prokaryotic communities associated with soil, roots, stems, and leaves. Soybean plants were sampled from replicated fields under long-term conventional, no-till, and organic management systems at three time points throughout the growing season. Results indicated that sample origin was the main driver of beta diversity in soybean-associated microbial communities, but management regime and plant growth stage were also significant factors. Similarly, differences in alpha diversity are driven by compartment and sample origin. Overall, the organic management system had lower fungal and bacterial Shannon diversity. In prokaryotic communities, aboveground tissues were dominated by Sphingomonas and Methylobacterium while belowground samples were dominated by Bradyrhizobium and Sphingomonas. Aboveground fungal communities were dominated by Davidiella across all management systems, while belowground samples were dominated by Fusarium and Mortierella. Specific taxa including potential plant beneficials such as Mortierella were indicator species of the conventional and organic management systems. No-till management increased the abundance of groups known to contain plant beneficial organisms such as Bradyrhizobium and Glomeromycotina. Network analyses show different highly connected hub taxa were present in each management system. Overall, this research demonstrates how specific long-term cropping management systems alter microbial communities and how those communities change throughout the growth of soybean.

Dataset Information

Molecular cloning and functional analysis of the promoter of ?-Tocopherol Methyl Transferase (?-TMT) gene of soybean (Glycine max).

Publications

Molecular cloning and functional analysis of the promoter of <i>γ-Tocopherol Methyl Transferase</i> (<i>γ-TMT</i>) gene of soybean (<i>Glycine max</i>).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets