Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line.
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ABSTRACT:
SUBMITTER: Kainulainen MH
PROVIDER: S-EPMC6050991 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line.
Kainulainen Markus H MH Nichol Stuart T ST Albariño César G CG Spiropoulou Christina F CF
The Journal of infectious diseases 20171201 11
Modern ebolavirus diagnostics rely primarily on quantitative reverse transcription-polymerase chain reaction (qRT-PCR), a sensitive method to detect viral genetic material in the acute phase of the disease. However, qRT-PCR does not confirm presence of infectious virus, presenting limitations in patient and outbreak management. Attempts to isolate infectious virus rely on in vivo or basic cell culture approaches, which prohibit rapid results and screening. In this study, we present a novel repor ...[more]
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