Ontology highlight
ABSTRACT:
SUBMITTER: Zhang B
PROVIDER: S-EPMC6051922 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Zhang Bojie B Cheng Ming M Rempel Don D Gross Michael L ML
Methods (San Diego, Calif.) 20180523
Fast photochemical oxidation of proteins (FPOP) is a footprinting technique used in mass spectrometry-based structural proteomics. It has been applied to solve a variety of problems in different areas of biology. A FPOP platform requires a laser, optics, and sample flow path properly assembled to enable fast footprinting. Sample preparation, buffer conditions, and reagent concentrations are essential to obtain reasonable oxidations on proteins. FPOP samples can be analyzed by LC-MS methods to me ...[more]