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Cell paintballing using optically targeted coacervate microdroplets.

ABSTRACT: We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the microdroplets make this novel "cell paintballing" technology a highly advantageous option for spatially-directed cell functionalization, with potential applications in single cell stimulation, transfection and differentiation.

SUBMITTER: Armstrong JPK

PROVIDER: S-EPMC6054073 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Cell paintballing using optically targeted coacervate microdroplets.

Armstrong James P K JPK Olof Sam N SN Jakimowicz Monika D MD Hollander Anthony P AP Mann Stephen S Davis Sean A SA Miles Mervyn J MJ Patil Avinash J AJ Perriman Adam W AW

Chemical science 20150720 11

We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished <i>via</i> active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet-membrane fusion. The facile preparation, high sequestration efficiency and inherent ...[more]

PMID: 30090225

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