Ontology highlight
ABSTRACT:
SUBMITTER: Ciulla DA
PROVIDER: S-EPMC6054137 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Ciulla Daniel A DA Jorgensen Michael T MT Giner José-Luis JL Callahan Brian P BP
Journal of the American Chemical Society 20170925 3
Proteins in the hedgehog family undergo self-catalyzed endoproteolysis involving nucleophilic attack by a molecule of cholesterol. Recently, a conserved aspartate residue (D303, or D46) of hedgehog was identified as the general base that activates cholesterol during this unusual autoprocessing event; mutation of the catalyzing functional group (D303A) reduces activity by >10<sup>4</sup>-fold. Here we report near total rescue of this ostensibly dead general base mutant by a synthetic substrate, 3 ...[more]