Dataset Information

Development of 16 microsatellite markers for the Korean endemic Vicia hirticalycina (Fabaceae).

ABSTRACT: Premise of the Study:Vicia hirticalycina (Fabaceae) is a narrowly endemic species restricted to mountain valleys of southern Korea. To investigate its fine-scale genetic diversity and differentiation in Korea, we developed polymorphic microsatellite markers. Methods and Results:Sixteen polymorphic microsatellite markers were developed from Illumina MiSeq data. In 74 individual plants from four populations, one to seven alleles were expressed for each locus. The levels of observed and expected heterozygosity ranged from 0.000 to 0.778 and from 0.000 to 0.738, respectively. Cross-amplification was conducted with three related species and seven to 11 markers were successfully amplified. Conclusions:These new microsatellite markers will be useful in future studies on the population genetics of V. hirticalycina.

SUBMITTER: Park JW

PROVIDER: S-EPMC6055556 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Development of 16 microsatellite markers for the Korean endemic <i>Vicia hirticalycina</i> (Fabaceae).

Park Jong-Won JW Choi In-Su IS Choi Byoung-Hee BH

Applications in plant sciences 20180601 7

<h4>Premise of the study</h4><i>Vicia hirticalycina</i> (Fabaceae) is a narrowly endemic species restricted to mountain valleys of southern Korea. To investigate its fine-scale genetic diversity and differentiation in Korea, we developed polymorphic microsatellite markers.<h4>Methods and results</h4>Sixteen polymorphic microsatellite markers were developed from Illumina MiSeq data. In 74 individual plants from four populations, one to seven alleles were expressed for each locus. The levels of ob ...[more]

PMID: 30131912

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Project description:Various plant species are endemic to the Korean Peninsula, but their evolutionary divergence and establishment are poorly understood. One of these, Lespedeza maritima, has been proposed as either a hybrid (L. cyrtobotrya × L. maximowiczii) or a synonym of L. thunbergii. A distinct taxon, L. uekii, has been proposed for inland populations. We investigated genetic diversity and structure in L. maritima and related taxa to resolve this. Genotypes of L. maritima (n = 244, including L. uekii) were determined using 12 microsatellite loci, then compared with those of related species. Genetic diversity within L. maritima was estimated, and Bayesian clustering analysis was used to represent its genetic structure and that of related taxa. Its distribution during the last glacial maximum (LGM) was predicted using ecological niche modelling (ENM). Neighbour-joining (NJ) analysis and principal coordinate analysis (PCoA) were used to investigate relationships among species. Bayesian tree based on nuclear ribosomal internal transcribed spacers (nrITS) was also reconstructed to show relationships and divergence time among species. Morphological features were examined using flower characteristics. In result, expected heterozygosity (H E) and allelic richness (A R) within L. maritima were higher in southern than northern populations. Bayesian clustering analysis largely assigned populations to two clusters (K = 2) (south vs. north). The ENM showed that L. maritima occurred around the East China Sea and Korean Strait land bridge during the LGM. Compared with other Lespedeza species, L. maritima was assigned to an independent cluster (K = 2-5), supported by the NJ, PCoA, Bayesian tree and morphological examination results. Lespedeza maritima and L. uekii were clustered to one clade on Bayesian tree. Given results, current L. maritima populations derive from post-LGM colonization away from southern refugia. The type L. uekii, which grows inland, is thought synonym of L. maritima. In addition, L. maritima is considered a distinct species, compared with related taxa.

Dataset Information

Development of 16 microsatellite markers for the Korean endemic Vicia hirticalycina (Fabaceae).

Publications

Development of 16 microsatellite markers for the Korean endemic <i>Vicia hirticalycina</i> (Fabaceae).

Similar Datasets

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