Developmental changes in the methylation status of regulatory elements in the murine alpha 1(I) collagen gene.

ABSTRACT: Regulatory elements contributing to the tissue-specific regulation of the murine alpha 1(I) collagen (Colla1) gene have previously been identified in its promoter region and first intron. Because several lines of evidence indicate that DNA methylation may be involved in the tissue-specific regulation of Colla1 gene expression, we have analyzed the methylation status of the 5' region of the gene by restriction analysis and a methylation-dependent PCR assay. All sites tested were unmethylated in sperm DNA. The region surrounding the start site of transcription was partially or completely methylated in undifferentiated embryonal cell lines, suggesting that it may be marked by de novo methylation during early embryonic development. In differentiated cells and adult tissues, the Colla1 promoter

SUBMITTER: Rhodes K 

PROVIDER: S-EPMC6057359 | biostudies-literature | 1992

REPOSITORIES: biostudies-literature