Ontology highlight
ABSTRACT:
SUBMITTER: DeWitt WS
PROVIDER: S-EPMC6057832 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
DeWitt William S WS Yu Krystle K Q KKQ Wilburn Damien B DB Sherwood Anna A Vignali Marissa M Day Cheryl L CL Scriba Thomas J TJ Robins Harlan S HS Swanson Willie J WJ Emerson Ryan O RO Bradley Philip H PH Seshadri Chetan C
Journal of immunology (Baltimore, Md. : 1950) 20180618 3
Human T cells that recognize lipid Ags presented by highly conserved CD1 proteins often express semi-invariant TCRs, but the true diversity of lipid Ag-specific TCRs remains unknown. We use CD1b tetramers and high-throughput immunosequencing to analyze thousands of TCRs from ex vivo-sorted or in vitro-expanded T cells specific for the mycobacterial lipid Ag, glucose monomycolate. Our results reveal a surprisingly diverse repertoire resulting from editing of germline-encoded gene rearrangements a ...[more]