Single-cell transcriptome analysis of lineage diversity in high-grade glioma.

ABSTRACT:

Background

Despite extensive molecular characterization, we lack a comprehensive understanding of lineage identity, differentiation, and proliferation in high-grade gliomas (HGGs).

Methods

We sampled the cellular milieu of HGGs by profiling dissociated human surgical specimens with a high-density microwell system for massively parallel single-cell RNA-Seq. We analyzed the resulting profiles to identify subpopulations of both HGG and microenvironmental cells and applied graph-based methods to infer structural features of the malignantly transformed populations.

Results

While HGG cells can resemble glia or even immature neurons and form branched lineage structures, mesenchymal transformation results in unstructured populations. Glioma cells in a subset of mesenchymal tu

SUBMITTER: Yuan J 

PROVIDER: S-EPMC6058390 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature