Co-extraction of genomic DNA & total RNA from recalcitrant woody tissues for next-generation sequencing studies.
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ABSTRACT:
SUBMITTER: Zeng Z
PROVIDER: S-EPMC6060392 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Co-extraction of genomic DNA & total RNA from recalcitrant woody tissues for next-generation sequencing studies.
Zeng Zhen Z Raffaello Tommaso T Liu Meng-Xia MX Asiegbu Fred O FO
Future science OA 20180425 6
The successful implementation of next-generation sequencing techniques in plant and woody tissues depends on the quality of initial starting material. This study demonstrated the use of a modified protocol that enabled the simultaneous extraction of both genomic DNA and total RNA from recalcitrant woody material. The genetic material obtained by this protocol is of high quality and can be directly used in downstream analysis (e.g., next-generation sequencing). This protocol is particularly usefu ...[more]
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