Identification of metabolic phenotypes in childhood obesity by 1H NMR metabolomics of blood plasma.
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ABSTRACT: Aim
To identify the plasma metabolic profile associated with childhood obesity and its metabolic phenotypes.Materials & methods
The plasma metabolic profile of 65 obese and 37 normal-weight children was obtained using proton NMR spectroscopy. NMR spectra were rationally divided into 110 integration regions, which reflect relative metabolite concentrations, and were used as statistical variables.Results
Obese children show increased levels of lipids, N-acetyl glycoproteins, and lactate, and decreased levels of several amino acids, α-ketoglutarate, glucose, citrate, and cholinated phospholipids as compared with normal-weight children. Metabolically healthy children show lower levels of lipids and lactate, and higher levels of several amino acids and cholinated phospholipids, as compared with unhealthy children.Conclusion
This study reveals new valuable findings in the field of metabolomics and childhood obesity. Although validation should be performed, the proof of principle looks promising and justifies a deeper investigation of the diagnostic possibilities of proton NMR metabolomics in follow-up studies. Trial registration: NCT03014856. Registered January 9, 2017.
SUBMITTER: Bervoets L
PROVIDER: S-EPMC6060399 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Identification of metabolic phenotypes in childhood obesity by <sup>1</sup>H NMR metabolomics of blood plasma.
Bervoets Liene L Massa Guy G Guedens Wanda W Reekmans Gunter G Noben Jean-Paul JP Adriaensens Peter P
Future science OA 20180523 6
<h4>Aim</h4>To identify the plasma metabolic profile associated with childhood obesity and its metabolic phenotypes.<h4>Materials & methods</h4>The plasma metabolic profile of 65 obese and 37 normal-weight children was obtained using proton NMR spectroscopy. NMR spectra were rationally divided into 110 integration regions, which reflect relative metabolite concentrations, and were used as statistical variables.<h4>Results</h4>Obese children show increased levels of lipids, N-acetyl glycoproteins ...[more]
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