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Application of Genome Editing Techniques in Immunology.

ABSTRACT: The idea of using the effector immune cells to specifically fight cancer has recently evolved into an exciting concept of adoptive cell therapies. Indeed, genetically engineered T cells expressing on their surface recombinant, cancer-targeted receptors have been shown to induce promising response in oncological patients. However, in addition to exogenous expression of such receptors, there is also a need for disruption of certain genes in the immune cells to achieve more potent disease-targeted actions, to produce universal chimeric antigen receptor-based therapies or to study the signaling pathways in detail. In this review, we present novel genetic engineering methods, mainly TALEN and CRISPR/Cas9 systems, that can be used for such purposes. These unique techniques may contribute to creating more successful immune therapies against cancer or prospectively other diseases as well.

SUBMITTER: Zych AO

PROVIDER: S-EPMC6061149 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Application of Genome Editing Techniques in Immunology.

Zych Agata O AO Bajor Malgorzata M Zagozdzon Radoslaw R

Archivum immunologiae et therapiae experimentalis 20180117 4

The idea of using the effector immune cells to specifically fight cancer has recently evolved into an exciting concept of adoptive cell therapies. Indeed, genetically engineered T cells expressing on their surface recombinant, cancer-targeted receptors have been shown to induce promising response in oncological patients. However, in addition to exogenous expression of such receptors, there is also a need for disruption of certain genes in the immune cells to achieve more potent disease-targeted ...[more]

PMID: 29344676

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