Dataset Information

Spin-manipulated nanoscopy for single nitrogen-vacancy center localizations in nanodiamonds.

ABSTRACT: Due to their exceptional optical and magnetic properties, negatively charged nitrogen-vacancy (NV^-) centers in nanodiamonds (NDs) have been identified as an indispensable tool for imaging, sensing and quantum bit manipulation. The investigation of the emission behaviors of single NV^- centers at the nanoscale is of paramount importance and underpins their use in applications ranging from quantum computation to super-resolution imaging. Here, we report on a spin-manipulated nanoscopy method for nanoscale resolutions of the collectively blinking NV^- centers confined within the diffraction-limited region. Using wide-field localization microscopy combined with nanoscale spin manipulation and the assistance of a microwave source tuned to the optically detected magnetic resonance (ODMR) frequency, we discovered that two collectively blinking NV^- centers can be resolved. Furthermore, when the collective emitters possess the same ground state spin transition frequency, the proposed method allows the resolving of each single NV^- center via an external magnetic field used to split the resonant dips. In spin manipulation, the three-level blinking dynamics provide the means to resolve two NV^- centers separated by distances of 23?nm. The method presented here offers a new platform for studying and imaging spin-related quantum interactions at the nanoscale with super-resolution techniques.

SUBMITTER: Barbiero M

PROVIDER: S-EPMC6062043 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Spin-manipulated nanoscopy for single nitrogen-vacancy center localizations in nanodiamonds.

Barbiero Martina M Castelletto Stefania S Gan Xiaosong X Gu Min M

Light, science & applications 20171103 11

Due to their exceptional optical and magnetic properties, negatively charged nitrogen-vacancy (NV<sup>-</sup>) centers in nanodiamonds (NDs) have been identified as an indispensable tool for imaging, sensing and quantum bit manipulation. The investigation of the emission behaviors of single NV<sup>-</sup> centers at the nanoscale is of paramount importance and underpins their use in applications ranging from quantum computation to super-resolution imaging. Here, we report on a spin-manipulated n ...[more]

PMID: 30167213

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Project description:The fluorescence intensity emitted by nitrogen-vacancy (NV) centers in diamond nanoparticles can be readily modulated by the application of a magnetic field using a small electromagnet. By acquiring interleaved images acquired in the presence and absence of the magnetic field and performing digital subtraction, the fluorescence intensity of the NV nanodiamond can be isolated from scattering and autofluorescence even when these backgrounds are changing monotonically during the experiments. This approach has the potential to enable the robust identification of nanodiamonds in organisms and other complex environments. Yet, the practical application of magnetic modulation imaging to realistic systems requires the use of quantitative analysis methods based on signal-to-noise considerations. Here, we describe the use of magnetic modulation to analyze the uptake of diamond nanoparticles from an aqueous environment into Caenorhabditis elegans, used here as a model system for identification and quantification of nanodiamonds in complex matrices. Based on the observed signal-to-noise ratio of sets of digitally subtracted images, we show that nanodiamonds can be identified on an individual pixel basis with a >99.95% confidence. To determine whether surface functionalization of the nanodiamond significantly impacted uptake, we used this approach to analyze the presence of nanodiamonds in C. elegans that had been exposed to these functionalized nanodiamonds in the water column, with uptake likely occurring by ingestion. In each case, the images show a significant nanoparticle uptake. However, differences in uptake between the three ligands were not outside of the experimental error, indicating that additional factors beyond the surface charge are important factors controlling uptake. Analysis of the number of pixels above the threshold in individual C. elegans organisms revealed distributions that deviate significantly from a Poisson distribution, suggesting that uptake of nanoparticles may not be a statistically independent event. The results presented here demonstrate that magnetic modulation combined with quantitative analysis of the resulting images can be used to robustly characterize nanoparticle uptake into organisms.

Dataset Information

Spin-manipulated nanoscopy for single nitrogen-vacancy center localizations in nanodiamonds.

Publications

Spin-manipulated nanoscopy for single nitrogen-vacancy center localizations in nanodiamonds.

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