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Mitochondrial impairment and oxidative stress mediated apoptosis induced by ?-Fe2O3 nanoparticles in Saccharomyces cerevisiae.

ABSTRACT: In this study, the potential toxicity of ?-Fe2O3-NPs was investigated using a unicellular eukaryote model, Saccharomyces cerevisiae (S. cerevisiae). The results showed that cell viability and proliferation were significantly decreased (p < 0.01) following exposure to 100-600 mg L-1 for 24 h. The IC50 and LC50 values were 352 and 541 mg L-1, respectively. Toxic effects were attributed to ?-Fe2O3-NPs rather than iron ions released from the NPs. ?-Fe2O3-NPs were accumulated in the vacuole and cytoplasm, and the maximum accumulation (3.95 mg g-1) was reached at 12 h. About 48.6% of cells underwent late apoptosis/necrosis at 600 mg L-1, and the mitochondrial transmembrane potential was significantly decreased (p < 0.01) at 50-600 mg L-1. Biomarkers of oxidative stress [reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)] and the expression of apoptosis-related genes (Yca1, Nma111, Nuc1 and SOD) were significantly changed after exposure. These combined results indicated that ?-Fe2O3-NPs were rapidly internalized in S. cerevisiae, and the accumulated NPs induced cell apoptosis mediated by mitochondrial impairment and oxidative stress.

SUBMITTER: Zhu S 

PROVIDER: S-EPMC6062213 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Mitochondrial impairment and oxidative stress mediated apoptosis induced by α-Fe<sub>2</sub>O<sub>3</sub> nanoparticles in <i>Saccharomyces cerevisiae</i>.

Zhu Song S   Luo Fei F   Zhu Bin B   Wang Gao-Xue GX  

Toxicology research 20170718 5

In this study, the potential toxicity of α-Fe<sub>2</sub>O<sub>3</sub>-NPs was investigated using a unicellular eukaryote model, <i>Saccharomyces cerevisiae</i> (<i>S. cerevisiae</i>). The results showed that cell viability and proliferation were significantly decreased (<i>p</i> < 0.01) following exposure to 100-600 mg L<sup>-1</sup> for 24 h. The IC<sub>50</sub> and LC<sub>50</sub> values were 352 and 541 mg L<sup>-1</sup>, respectively. Toxic effects were attributed to α-Fe<sub>2</sub>O<sub>3  ...[more]

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