Four Key Steps Control Glycolytic Flux in Mammalian Cells.
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ABSTRACT:
SUBMITTER: Tanner LB
PROVIDER: S-EPMC6062487 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Four Key Steps Control Glycolytic Flux in Mammalian Cells.
Tanner Lukas Bahati LB Goglia Alexander G AG Wei Monica H MH Sehgal Talen T Parsons Lance R LR Park Junyoung O JO White Eileen E Toettcher Jared E JE Rabinowitz Joshua D JD
Cell systems 20180627 1
Altered glycolysis is a hallmark of diseases including diabetes and cancer. Despite intensive study of the contributions of individual glycolytic enzymes, systems-level analyses of flux control through glycolysis remain limited. Here, we overexpress in two mammalian cell lines the individual enzymes catalyzing each of the 12 steps linking extracellular glucose to excreted lactate, and find substantial flux control at four steps: glucose import, hexokinase, phosphofructokinase, and lactate export ...[more]
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