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Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities.

ABSTRACT: LK-3, an amphipathic dimeric peptide linked by two disulfide bonds, and related isomeric bundles were synthesized, and their cell penetrating abilities were investigated. The measurements using size exclusion chromatography and dynamic light scattering show that LK-3 and its isomers form cell penetrating oligomers. Calculations, performed for various types of peptide isomers, elucidate a strong correlation between the amphipathic character of dimers and cell penetration ability. The results suggest that the amphipathicities of LK-3 and related bundle dimers are responsible for their oligomerization propensities which in turn determine their cell penetrating abilities. The observations made in this study provide detailed information about the mechanism of cell uptake of LK-3 and suggest a plausible insight of the early stage of nanoparticle formation of the cell penetrating amphipathic peptides.

SUBMITTER: Hyun S

PROVIDER: S-EPMC6062827 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities.

Hyun Soonsil S Lee Yuno Y Jin Sun Mi SM Cho Jane J Park Jeemin J Hyeon Changbong C Kim Key-Sun KS Lee Yan Y Yu Jaehoon J

ACS central science 20180710 7

LK-3, an amphipathic dimeric peptide linked by two disulfide bonds, and related isomeric bundles were synthesized, and their cell penetrating abilities were investigated. The measurements using size exclusion chromatography and dynamic light scattering show that LK-3 and its isomers form cell penetrating oligomers. Calculations, performed for various types of peptide isomers, elucidate a strong correlation between the amphipathic character of dimers and cell penetration ability. The results sugg ...[more]

PMID: 30062117

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