State-dependent cell-type-specific membrane potential dynamics and unitary synaptic inputs in awake mice.

ABSTRACT: The cellular and synaptic mechanisms driving cell-type-specific function during various cortical network activities and behaviors are poorly understood. Here, we targeted whole-cell recordings to two classes of inhibitory GABAergic neurons in layer 2/3 of the barrel cortex of awake head-restrained mice and correlated spontaneous membrane potential dynamics with cortical state and whisking behavior. Using optogenetic stimulation of single layer 2/3 excitatory neurons we measured unitary excitatory postsynaptic potentials (uEPSPs) across states. During active states, characterized by whisking and reduced low-frequency activity in the local field potential, parvalbumin-expressing neurons depolarized and, albeit in a small number of recordings, received uEPSPs with increased amplitude. In cont

SUBMITTER: Pala A 

PROVIDER: S-EPMC6063730 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature