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Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress.

ABSTRACT: Upregulation of histone acetylation plays a critical role in the dysregulation of transcription. It alters the structure of chromatin, which leads to the onset of cancer. Histone deacetylase inhibitors may therefore be a promising way to limit cancer progression. In this study, we examined the effects of droxinostat on the growth of HT-29 colon cancer cells. Our results show that droxinostat effectively inhibited cell growth and colony-forming ability by inducing cellular apoptosis and ROS production in HT-29 cells. Notably, the apoptotic inhibitor Z-VAD-FMK significantly decreased the levels of cellular apoptosis and the antioxidant ?-tocotrienol (GT3) significantly decreased ROS production induced by droxinostat treatment. Z-VAD-FMK and GT3 also partially reversed the negative growth effects of droxinstat on HT-29 cells. GT3 treatment decreased cellular apoptosis and increased colony-forming ability upon droxinostat administration. Z-VAD-FMK treatment also partially decreased droxinostat-induced ROS production. Our findings suggest that the effects of droxinostat on colon cancer cells are mediated by the induction of oxidative stress and apoptotic cell death.

SUBMITTER: Huang Y

PROVIDER: S-EPMC6064062 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress.

Huang Ying Y Yang Wuping W Zeng Huihong H Hu Chuan C Zhang Yaqiong Y Ding Nanhua N Fan Guangqin G Shao Lijian L Kuang Bohai B

Cellular & molecular biology letters 20180728

Upregulation of histone acetylation plays a critical role in the dysregulation of transcription. It alters the structure of chromatin, which leads to the onset of cancer. Histone deacetylase inhibitors may therefore be a promising way to limit cancer progression. In this study, we examined the effects of droxinostat on the growth of HT-29 colon cancer cells. Our results show that droxinostat effectively inhibited cell growth and colony-forming ability by inducing cellular apoptosis and ROS produ ...[more]

PMID: 30065760

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Dataset Information

Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress.

Publications

Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress.

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