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Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes.

ABSTRACT: Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER^T2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.

SUBMITTER: Ransom RC

PROVIDER: S-EPMC6065311 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes.

Ransom Ryan C RC Foster Deshka S DS Salhotra Ankit A Jones Ruth Ellen RE Marshall Clement D CD Leavitt Tripp T Murphy Matthew P MP Moore Alessandra L AL Blackshear Charles P CP Brett Elizabeth A EA Wan Derrick C DC Longaker Michael T MT

Nature communications 20180730 1

Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreER<sup>T2</sup> recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre driver ...[more]

PMID: 30061668

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