Ontology highlight
ABSTRACT:
SUBMITTER: Han Y
PROVIDER: S-EPMC6066478 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Han Yan Y Yan Chunli C Fishbain Susan S Ivanov Ivaylo I He Yuan Y
Cell discovery 20180731
RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III-TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and P ...[more]