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A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions.

ABSTRACT: Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLC? that regulate the activation of PKD at trans-Golgi membranes. We further show that this molecular network coordinates the formation of TGN-derived Rab6-positive transport carriers delivering cargo for localized exocytosis at focal adhesions.

SUBMITTER: Eisler SA

PROVIDER: S-EPMC6070338 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions.

Eisler Stephan A SA Curado Filipa F Link Gisela G Schulz Sarah S Noack Melanie M Steinke Maren M Olayioye Monilola A MA Hausser Angelika A

eLife 20180720

Protein kinase D (PKD) is a family of serine/threonine kinases that is required for the structural integrity and function of the Golgi complex. Despite its importance in the regulation of Golgi function, the molecular mechanisms regulating PKD activity are still incompletely understood. Using the genetically encoded PKD activity reporter G-PKDrep we now uncover a Rho signaling network comprising GEF-H1, the RhoGAP DLC3, and the Rho effector PLCε that regulate the activation of PKD at trans-Golgi ...[more]

PMID: 30028295

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