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Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.

ABSTRACT: Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-?m-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-? filaments in a mouse model of Alzheimer's disease.

SUBMITTER: Mlodzianoski MJ

PROVIDER: S-EPMC6071422 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.

Mlodzianoski Michael J MJ Cheng-Hathaway Paul J PJ Bemiller Shane M SM McCray Tyler J TJ Liu Sheng S Miller David A DA Lamb Bruce T BT Landreth Gary E GE Huang Fang F

Nature methods 20180716 8

Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filame ...[more]

PMID: 30013047

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